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Di-methyl-Histone H3...

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Di-methyl-Histone H3.1(K4)Antibody

  恒遠(yuǎn)生物抗體平臺現(xiàn)已擁有4萬多種抗體(含單抗和多抗),包括人類常用抗體,模式生物類相關(guān)抗體、小分子抗體、二抗、標(biāo)簽抗體及內(nèi)參抗體等。并以每年4000種的速度在持續(xù)增長。

  恒遠(yuǎn)生物擁有專業(yè)的質(zhì)控團(tuán)隊(duì),配備先進(jìn)實(shí)驗(yàn)儀器,現(xiàn)已成功地建立了ChIP平臺,因此我們的抗體不僅能提供Biotin、FITC、HRP等標(biāo)記,大部分抗體都能進(jìn)行ELISA、WB、IHC、IF、IP、ChIP等應(yīng)用驗(yàn)證。

  我們致力于為所有用戶提供高親和力、高特異性的優(yōu)質(zhì)抗體,歡迎來電咨詢。

Di-methyl-Histone H3.1(K4)Antibody

 
  • 貨號:
    HYK-200099
  • 規(guī)格:
     
  • 圖片:
    • Western Blot
      Positive WB detected in:Mouse brain tissue,Mouse heart tissue
      All lanes:Di-methyl-Histone H3.1(K4)antibody at 0.55μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 15 KDa
      Observed band size: 15 KDa
    • Immunocytochemistry analysis of CSB-RA010418A04me2HU diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-RA010418A04me2HU at 1:34,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
    • Overlay histogram showing Hela cells stained with CSB-RA010418A04me2HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

產(chǎn)品詳情

  • Uniprot No.:
    P68431
  • 基因名:
    HIST1H3A
  • 別名:
    H3 histone family member E pseudogene antibody; H3 histone family; member A antibody; H3/A antibody; H31_HUMAN antibody; H3F3 antibody; H3FA antibody; Hist1h3a antibody; HIST1H3B antibody; HIST1H3C antibody; HIST1H3D antibody; HIST1H3E antibody; HIST1H3F antibody; HIST1H3G antibody; HIST1H3H antibody; HIST1H3I antibody; HIST1H3J antibody; HIST3H3 antibody; histone 1; H3a antibody; Histone cluster 1; H3a antibody; Histone H3 3 pseudogene antibody; Histone H3.1 antibody; Histone H3/a antibody; Histone H3/b antibody; Histone H3/c antibody; Histone H3/d antibody; Histone H3/f antibody; Histone H3/h antibody; Histone H3/i antibody; Histone H3/j antibody; Histone H3/k antibody; Histone H3/l antibody
  • 反應(yīng)種屬:
    Human, Mouse
  • 免疫原:
    A synthesized peptide
  • 免疫原種屬:
    Homo sapiens (Human)
  • 標(biāo)記方式:
    Non-conjugated
  • 克隆類型:
    Monoclonal
  • 抗體亞型:
    Rabbit IgG
  • 純化方式:
    Affinity-chromatography
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式:
    Liquid
  • 應(yīng)用范圍:
    ELISA, WB, ICC, IF, FC
  • 推薦稀釋比:
    Application Recommended Dilution
    WB 1:500-1:2000
    ICC 1:50-1:500
    IF 1:30-1:200
  • Protocols:
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunofluorescence (IF) Protocol
    Flow cytometry (FC) Protocol
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.


N Antibody

N Antibody, HRP conjugated

S Antibody, HRP conjugated

S Antibody, Biotin conjugated

SARS-CoV-2 Spike RBD Nanobody

N Antibody


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