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PODXL Monoclonal Ant...

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PODXL Monoclonal Antibody

  恒遠生物抗體平臺現(xiàn)已擁有4萬多種抗體(含單抗和多抗),包括人類常用抗體,模式生物類相關抗體、小分子抗體、二抗、標簽抗體及內參抗體等。并以每年4000種的速度在持續(xù)增長。

  恒遠生物擁有專業(yè)的質控團隊,配備先進實驗儀器,現(xiàn)已成功地建立了ChIP平臺,因此我們的抗體不僅能提供Biotin、FITC、HRP等標記,大部分抗體都能進行ELISA、WB、IHC、IF、IP、ChIP等應用驗證。

  我們致力于為所有用戶提供高親和力、高特異性的優(yōu)質抗體。

PODXL Monoclonal Antibody

 
  • 貨號:
    HYK-200031
  • 規(guī)格:
     
  • 圖片:
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate
      All lanes: PODXL antibody at 2.5μg/ml
      Secondary
      Goat polyclonal to Mouse IgG at 1/5000 dilution
      Predicted band size: 59, 56 kDa
      Observed band size: 59 kDa

    • Western Blot
      Positive WB detected in: A549 whole cell lysate
      All lanes: PODXL antibody at 2.5μg/ml
      Secondary
      Goat polyclonal to Mouse IgG at 1/5000 dilution
      Predicted band size: 59, 56 kDa
      Observed band size: 59 kDa

    • Western Blot
      Positive WB detected in: Mouse brain tissue
      All lanes: PODXL antibody at 0.43μg/ml
      Secondary
      Goat polyclonal to Mouse IgG at 1/5000 dilution
      Predicted band size: 59, 56 kDa
      Observed band size: 59 kDa

    • Western Blot
      Positive WB detected in: Rat brain tissue, Rat heart tissue, Rat kidney tissue
      All lanes: PODXL antibody at 1.28μg/ml
      Secondary
      Goat polyclonal to Mouse IgG at 1/5000 dilution
      Predicted band size: 59, 56 kDa
      Observed band size: 59 kDa

    • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-MA134471A0m at dilution of 1:200

    • Immunohistochemistry of paraffin-embedded human melanoma using CSB-MA134471A0m at dilution of 1:200

    • Immunofluorescent analysis of Hela cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

    • Immunofluorescent analysis of PC-3 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

    • Immunofluorescent analysis of A549 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

    • Immunofluorescent analysis of Ntera-2 cells using CSB-MA134471A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

    • Overlay histogram showing Hela cells stained with CSB-MA134471A0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10μg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IGg2b (10μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

產品詳情

  • 產品名稱:
    Mouse anti-Homo sapiens (Human) PODXL Monoclonal antibody
  • Uniprot No.:
    O00592
  • 基因名:
    PODXL
  • 別名:
    GCTM-2 antigen antibody; Gp2 antibody; Gp200 antibody; MGC138240 antibody; PC antibody; PCLP antibody; PCLP-1 antibody; PCLP1 antibody; Pcx antibody; Podocalyxin antibody; Podocalyxin like antibody; Podocalyxin like protein antibody; Podocalyxin-like protein 1 antibody; Podxl antibody; PODXL_HUMAN antibody
  • 宿主:
    Mouse
  • 反應種屬:
    Human, Mouse, Rat
  • 免疫原:
    Recombinant Human PODXL protein
  • 免疫原種屬:
    Homo sapiens (Human)
  • 標記方式:
    Non-conjugated
  • 克隆類型:
    Monoclonal
  • 抗體亞型:
    IgG2b
  • 純化方式:
    >95%, Protein G purified
  • 克隆號:
    4D2G7
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式:
    Liquid
  • 應用范圍:
    ELISA, WB, IHC, IP, IF, FC
  • 推薦稀釋比:
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:500
    IF 1:50-1:200
    IP 1:200-1:2000
  • Protocols:
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunohistochemistry (IHC) Protocol
    Immunoprecipitation (IP) Protocol
    Immunofluorescence (IF) Protocol
    Flow cytometry (FC) Protocol
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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